Prof. Dr. Jochen Staiger
Prof. Dr. Bernhard Reuss
Gabriele Schmidt
Dr. rer. nat. Julien Guy
Dr. rer. nat. Martin Möck
Dr. med. Rebeka Andrea Palicz
Dr. Stefan Pommer
Dr. rer. nat. Joachim Rosenbusch
Dr. rer. nat. Mirko Witte
Merve Özgür Erat
Aybeniz Ece Cetin
Xiaoyi Mao
Felix Preuss
Jenifer Rachel
Harun Akkoyun
Felicita Fischer
Philipp Kolligs
Lukas Müller
Flore Schork
Sophia Heidenreich
Ima Mansori
Leander Matthes
Paul Molis
Sandra Heinzl
Sabrina Hübner
Patricia Sprysch
Pavel Truschow
Dr. rer. nat. Csaba Dávid
Dr. rer. nat. Alvar Prönneke
PD Dr. Michael Rickmann
Dr. Marcel Ruiz Mejias
Dr. rer. nat. Dirk Schubert
Dr. Godwin Sokpor
Dr. rer. nat. Nidhi Subhashini
Dr. rer. nat. Tran Tuoc
Dr. med. Robin Wagener
Dr. rer. nat. Yuanbin Xie
Xiaojuan Zhou
Eman Abbas
Weilin Chen
Michael Feyerabend
Georg Hafner
Kamila Kiszka
Anouk Meeuwissen
Nieves Mingo Moreno
Ramanathan Narayanan
Huong Nguyen
Pauline Antonie Ulmke
Florian Walker
Khatuna Aslanishvili
Christina Bachmann
Simon Badura
Thore Behrendt
Jürgen Delchmann
Esther Alexandra Dockhorn
Tatjana Fischer
Anna Garcia Galera
Kristina Glöckner
Janis Hülsemann
Dilbrin Khelo
Stephen Olt
Bettina Pater
Alina Rüppel
Alexandra Sachkova
Bianca Scheuer
Lisa Thiecke
Joris Brehmer
Dennis Dalügge
Julia Dziubek
Ricardo Castro Hernandez
Fernando Gonzalez Ibanez
Christin Korb
Anette Mertens
Megha Patwa
Adrián Villalobos
Simon Weiler
Maxim Wintergoller
Nicolas Zdun
Anna Dudek
Heike Faust
Sabrina Heide
Ansgar Jahn
Linh Pham

Narayanan
Last Name: | Narayanan | Position: | PhD Student |
First Name: | Ramanathan | Ort: | Göttingen |
Akademischer Titel: | Tel.: |
Lebenslauf
- Since June 2013
PhD student at the Institute of Neuroanatomy, Centre for Anatomy, University Medical Centre Goettingen (UMG) in the research group of Dr. Tran Tuoc (Director: Prof. Dr. Jochen Staiger)
- 2012-13 Master’s thesis
‘’Development of a novel biosensor to detect glucocorticoid signalling and stress response in vivo in zebrafish larvae’’. Thesis advisor: Dr. Soojin Ryu, Developmental Genetics of the Nervous system, Max Planck Institute for Medical Research, Heidelberg, Germany
- 2011-13 Master’s in Neuroscience
International Max Planck Research School (IMPRS) for Neurosciences, Georg-August University of Goettingen, Germany
- 2010-11 Research Project Assistant
Laboratory of Dr. Soumya Iyengar, Systems Neuroscience division, National Brain Research Centre, Manesar, India
- 2006-10 Bachelor’s (Hons.) in Biotechnology
Shanmugha Arts Science Technology & Research Academy (SASTRA) University, Thanjavur, India
Forschung
In mammals, evolution of the neocortex is considered to have significantly contributed to higher cognitive functions. Mammalian cortex, a highly specialized six-layered structure, is further divided into distinct sensory, motor and association areas. Interestingly, the size and complexity of the cortex is achieved by neural progenitor cells of the ventricular (VZ) and subventricular zones (SVZ) early in development. In the background of recent evidences suggesting the role of mSWI/SNF (or BAF) complexes in cortical neurogenesis, I am interested to further investigate the role of BAF complex and its interaction partners in regulating cortical development in mouse.
Publikationen
2021
Mapping of domain-mediated protein-protein interaction by SPOT peptide assay.
Xiaoyi Mao, Godwin Sokpor, Jochen F Staiger, Huu Phuc Nguyen, Tran Tuoc.
STAR Protocols, Volume 2, Issue 2, 100503, 2021.
link
2019
RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.
Xie Y*, Hernandez RC*, Sokpor G, Pham L, Narayanan R, Rosenbusch J, Staiger JF, Tuoc T.
Molecular Neurobiology, https://doi.org/10.1007/s12035-019-1595-1, 2019.
abstract link
Chromatin remodeling factor BAF155 is an important regulator of many biological processes. As a core and scaffold subunit of the BAF (SWI/SNF-like) complex, BAF155 is capable of regulating the stability and function of the BAF complex. The spatiotemporal expression of BAF155 during embryogenesis is essential for various aspects of organogenesis, particularly in the brain development. However, our understanding of the mechanisms that regulate the expression and function of BAF155 is limited. Here, we report that RBM15, a subunit of the m6A methyltransferase complex, interacts with BAF155 mRNA and mediates BAF155 mRNA degradation through the mRNA methylation machinery. Ablation of endogenous RBM15 expression in cultured neuronal cells and in the developing cortex augmented the expression of BAF155. Conversely, RBM15 overexpression decreased BAF155 mRNA and protein levels, and perturbed BAF155 functions in vivo, including repression of BAF155-dependent transcriptional activity and delamination of apical radial glial progenitors as a hallmark of basal radial glial progenitor genesis. Furthermore, we demonstrated that the regulation of BAF155 by RBM15 depends on the activity of the mRNA methylation complex core catalytic subunit METTL3. Altogether, our findings reveal a new regulatory avenue that elucidates how BAF complex subunit stoichiometry and functional modulation are achieved in mammalian cells.
2018
Chromatin remodeling BAF155 subunit regulates the genesis of basal progenitors in developing cortex .
Narayanan R, Pham L, Kerimoglu C, Watanabe T, Hernandez RC, Sokpor G, Ulmke PA, Kiszka KA, Tonchev AB, Rosenbusch J, Seong RH, Teichmann U, Frahm J, Fischer F, Bonn S, Stoykova A, Staiger JF, Tuoc T.
iScience (Cell Press), DOI: https://doi.org/10.1016/j.isci.2018.05.014, 2018.
abstract link
The abundance of basal progenitors (BPs) - basal radial glia progenitors (bRGs) and basal intermediate progenitors (bIPs), in primate brain has been correlated to the high degree of cortical folding. Here we examined the role of BAF155, a subunit of the chromatin remodeling BAF complex, in generation of cortical progenitor heterogeneity. The conditional deletion of BAF155 led to diminished bIP pool and increased number of bRGs, due to delamination of apical RGs. We found that BAF155 is required for normal activity of neurogenic transcription factor PAX6, thus controlling expression of genes that are involved in bIP specification, cell-cell interaction and establishment of adherens junction. In PAX6-dependent manner, BAF155 regulates the expression of the CDC42 effector protein CEP4, thereby controlling progenitor delamination. Furthermore, BAF155-dependent chromatin remodeling seems to exert a specific role in the genesis of BPs through regulation of human RG-specific genes (such as Foxn4) that possibly acquired evolutionary significance.
2015
Loss of BAF (mSWI/SNF) complexes causes global transcriptional and chromatin state changes in forebrain development.
Ramanathan Narayanan, Mehdi Pirouz, Cemil Kerimoglu, Linh Pham, Robin J. Wagener, Kamila A. Kiszka, Joachim Rosenbusch, Michael Kessel, Andre Fischer, Anastassia Stoykova, Jochen F. Staiger, and Tran Tuoc.
Cell Reports, 2015.
13, 1–13
abstract link
BAF (Brg/Brm-associated factors) complexes play important roles in development and are linked to chromatin plasticity at selected genomic loci. Nevertheless, a full understanding of their role in development and chromatin remodeling has been hindered by the absence of mutants completely lacking BAF complexes. Here, we report that the loss of BAF155/BAF170 in double-conditional knock-out (dcKO) mice eliminates all known BAF subunits, resulting in an overall reduction in active chromatin marks (H3K9Ac), a global increase in repressive marks (H3K27me2/3), and down-regulation of gene expression. We demonstrate that BAF complexes interact with H3K27 demethylases (JMJD3, UTX) and potentiate their activity. Importantly BAF complexes are indispensable for forebrain development, including proliferation, differentiation and cell survival of neural progenitor cells. Our findings reveal a molecular mechanism mediated by BAF complexes that controls global transcriptional program and chromatin state in development.
2014
Roles of chromatin remodeling BAF complex in neural differentiation and reprogramming.
Narayanan R and Tuoc TC .
Cell and Tissue Research (DOI: 10.1007/s00441-013-1791-7), 2014.
abstract link
ATP-dependent BAF chromatin remodeling complexes play an essential role in the maintenance of the gene expression program by regulating the structure of chromatin. There is increasing evidence that BAF complexes based on the alternative ATPase subunits, Brg1 and Brm, control the differentiation of neural stem cells (NSCs) to generate distinct neural cell types and modulate trans-differentiation between cell types. The BAF complexes have dedicated functions at different stages of neural differentiation that appear to arise by combinatorial assembly of their subunits. Furthermore, the differentiation of NSCs is regulated by the tight interactions between the BAF chromatin remodeling complex and the transcriptional machinery. Here, we review recent insights into the functional interaction between BAF complexes and various transcription factors (TFs) in neural differentiation and cellular reprogramming
2013
BAF chromatin remodeling complex: Cortical size regulation and beyond.
Tuoc TC*, Narayanan R, Stoykova A*.
Cell Cycle, 12(8), 2013.
abstract link
The multi-subunit chromatin remodeling BAF complex controls different developmental processes. Using cortex-specific conditional knockout and overexpression mouse models, we have recently reported that BAF170, a subunit of the vertebrate BAF chromatin remodeling complex, interacts with transcription factor (TF) Pax6 to control cortical size and volume. The mechanistic basis includes suppression of the expression of Pax6 target genes, which are required for genesis of cortical intermediate progenitors (IPs) and specification of late neuronal subtype identity. In addition, we showed that a dynamic competition between BAF170 and BAF155 subunits within the BAF complex during progression of neurogenesis is a primary event in modulating the size of the mammalian cortex. Here, we present additional insights into the interaction between the BAF complex and TF Pax6 in the genesis of IPs of the developing cortex. Furthermore, we show that such competition between BAF170 and BAF155 is involved as well in the determination of the size of the embryonic body. Our results add new insights into a cell-intrinsic mechanism, mediated by the chromatin remodeling BAF complex, that controls vertebrate body shape and size.