Prof. Dr. Jochen Staiger
Prof. Dr. Bernhard Reuss
Gabriele Schmidt
Dr. rer. nat. Julien Guy
Dr. rer. nat. Martin Möck
Dr. med. Rebeka Andrea Palicz
Dr. Stefan Pommer
Dr. rer. nat. Joachim Rosenbusch
Dr. rer. nat. Mirko Witte
Merve Özgür Erat
Aybeniz Ece Cetin
Xiaoyi Mao
Felix Preuss
Jenifer Rachel
Harun Akkoyun
Felicita Fischer
Philipp Kolligs
Lukas Müller
Flore Schork
Sophia Heidenreich
Christin Korb
Ima Mansori
Leander Matthes
Paul Molis
Nicolas Zdun
Sandra Heinzl
Sabrina Hübner
Patricia Sprysch
Pavel Truschow
Dr. rer. nat. Csaba Dávid
Dr. rer. nat. Alvar Prönneke
PD Dr. Michael Rickmann
Dr. Marcel Ruiz Mejias
Dr. rer. nat. Dirk Schubert
Dr. Godwin Sokpor
Dr. rer. nat. Nidhi Subhashini
Dr. rer. nat. Tran Tuoc
Dr. med. Robin Wagener
Dr. rer. nat. Yuanbin Xie
Dr. rer. nat. Xiaojuan Zhou
Eman Abbas
Weilin Chen
Michael Feyerabend
Georg Hafner
Kamila Kiszka
Anouk Meeuwissen
Nieves Mingo Moreno
Ramanathan Narayanan
Huong Nguyen
Pauline Antonie Ulmke
Florian Walker
Khatuna Aslanishvili
Christina Bachmann
Simon Badura
Thore Behrendt
Jürgen Delchmann
Esther Alexandra Dockhorn
Tatjana Fischer
Anna Garcia Galera
Kristina Glöckner
Janis Hülsemann
Dilbrin Khelo
Stephen Olt
Bettina Pater
Alina Rüppel
Alexandra Sachkova
Bianca Scheuer
Lisa Thiecke
Joris Brehmer
Dennis Dalügge
Julia Dziubek
Ricardo Castro Hernandez
Fernando Gonzalez Ibanez
Anette Mertens
Megha Patwa
Adrián Villalobos
Simon Weiler
Maxim Wintergoller
Anna Dudek
Heike Faust
Sabrina Heide
Ansgar Jahn
Linh Pham

Hernandez
Last Name: | Hernandez | Position: | Master/Bachelor Student |
First Name: | Ricardo Castro | Location: | |
Academic Title: | Tel.: |
Curriculum Vitae
Publications
2019
RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.
Xie Y*, Hernandez RC*, Sokpor G, Pham L, Narayanan R, Rosenbusch J, Staiger JF, Tuoc T.
Molecular Neurobiology, https://doi.org/10.1007/s12035-019-1595-1, 2019.
abstract link
Chromatin remodeling factor BAF155 is an important regulator of many biological processes. As a core and scaffold subunit of the BAF (SWI/SNF-like) complex, BAF155 is capable of regulating the stability and function of the BAF complex. The spatiotemporal expression of BAF155 during embryogenesis is essential for various aspects of organogenesis, particularly in the brain development. However, our understanding of the mechanisms that regulate the expression and function of BAF155 is limited. Here, we report that RBM15, a subunit of the m6A methyltransferase complex, interacts with BAF155 mRNA and mediates BAF155 mRNA degradation through the mRNA methylation machinery. Ablation of endogenous RBM15 expression in cultured neuronal cells and in the developing cortex augmented the expression of BAF155. Conversely, RBM15 overexpression decreased BAF155 mRNA and protein levels, and perturbed BAF155 functions in vivo, including repression of BAF155-dependent transcriptional activity and delamination of apical radial glial progenitors as a hallmark of basal radial glial progenitor genesis. Furthermore, we demonstrated that the regulation of BAF155 by RBM15 depends on the activity of the mRNA methylation complex core catalytic subunit METTL3. Altogether, our findings reveal a new regulatory avenue that elucidates how BAF complex subunit stoichiometry and functional modulation are achieved in mammalian cells.
2018
Chromatin remodeling BAF155 subunit regulates the genesis of basal progenitors in developing cortex .
Narayanan R, Pham L, Kerimoglu C, Watanabe T, Hernandez RC, Sokpor G, Ulmke PA, Kiszka KA, Tonchev AB, Rosenbusch J, Seong RH, Teichmann U, Frahm J, Fischer F, Bonn S, Stoykova A, Staiger JF, Tuoc T.
iScience (Cell Press), DOI: https://doi.org/10.1016/j.isci.2018.05.014, 2018.
abstract link
The abundance of basal progenitors (BPs) - basal radial glia progenitors (bRGs) and basal intermediate progenitors (bIPs), in primate brain has been correlated to the high degree of cortical folding. Here we examined the role of BAF155, a subunit of the chromatin remodeling BAF complex, in generation of cortical progenitor heterogeneity. The conditional deletion of BAF155 led to diminished bIP pool and increased number of bRGs, due to delamination of apical RGs. We found that BAF155 is required for normal activity of neurogenic transcription factor PAX6, thus controlling expression of genes that are involved in bIP specification, cell-cell interaction and establishment of adherens junction. In PAX6-dependent manner, BAF155 regulates the expression of the CDC42 effector protein CEP4, thereby controlling progenitor delamination. Furthermore, BAF155-dependent chromatin remodeling seems to exert a specific role in the genesis of BPs through regulation of human RG-specific genes (such as Foxn4) that possibly acquired evolutionary significance.
ATP-dependent chromatin remodeling during cortical neurogenesis.
Sokpor G*, Hernandez RC*, Rosenbusch J, Staiger JF, Tuoc T.
Front. Neurosci. | doi: 10.3389/fnins.2018.00226 , 2018.
abstract link
The generation of individual neurons (neurogenesis) during cortical development occurs in discrete steps that are subtly regulated and orchestrated to ensure normal histogenesis and function of the cortex. Notably, various gene expression programs are known to critically drive many facets of neurogenesis with a high level of specificity during brain development. Typically, precise regulation of gene expression patterns ensures that key events like proliferation and differentiation of neural progenitors, specification of neuronal subtypes, as well as migration and maturation of neurons in the developing cortex occur properly. ATP-dependent chromatin remodeling complexes regulate gene expression through utilization of energy from ATP hydrolysis to reorganize chromatin structure. These chromatin remodeling complexes are characteristically multimeric, with some capable of adopting functionally distinct conformations via subunit reconstitution to perform specific roles in major aspects of cortical neurogenesis. In this review, we highlight the functions of such chromatin remodelers during cortical development. We also bring together various proposed mechanisms by which ATP-dependent chromatin remodelers function individually or in concert, to specifically modulate vital steps in cortical neurogenesis.