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Prof. Dr. Jochen Staiger

Prof. Dr. Bernhard Reuss

Gabriele Schmidt

Dr. rer. nat. Julien Guy

Dr. rer. nat. Martin Möck

Dr. med. Rebeka Andrea Palicz

Dr. Stefan Pommer

Dr. rer. nat. Joachim Rosenbusch

Dr. rer. nat. Mirko Witte

Merve Özgür Erat

Aybeniz Ece Cetin

Xiaoyi Mao

Felix Preuss

Jenifer Rachel

Harun Akkoyun

Felicita Fischer

Philipp Kolligs

Lukas Müller

Flore Schork

Sophia Heidenreich

Christin Korb

Ima Mansori

Leander Matthes

Paul Molis

Nicolas Zdun

Sandra Heinzl

Sabrina Hübner

Patricia Sprysch

Pavel Truschow

Dr. rer. nat. Csaba Dávid

Dr. rer. nat. Alvar Prönneke

PD Dr. Michael Rickmann

Dr. Marcel Ruiz Mejias

Dr. rer. nat. Dirk Schubert

Dr. Godwin Sokpor

Dr. rer. nat. Nidhi Subhashini

Dr. rer. nat. Tran Tuoc

Dr. med. Robin Wagener

Dr. rer. nat. Yuanbin Xie

Dr. rer. nat. Xiaojuan Zhou

Eman Abbas

Weilin Chen

Michael Feyerabend

Georg Hafner

Kamila Kiszka

Anouk Meeuwissen

Nieves Mingo Moreno

Ramanathan Narayanan

Huong Nguyen

Pauline Antonie Ulmke

Florian Walker

Khatuna Aslanishvili

Christina Bachmann

Simon Badura

Thore Behrendt

Jürgen Delchmann

Esther Alexandra Dockhorn

Tatjana Fischer

Anna Garcia Galera

Kristina Glöckner

Janis Hülsemann

Dilbrin Khelo

Stephen Olt

Bettina Pater

Alina Rüppel

Alexandra Sachkova

Bianca Scheuer

Lisa Thiecke

Joris Brehmer

Dennis Dalügge

Julia Dziubek

Ricardo Castro Hernandez

Fernando Gonzalez Ibanez

Anette Mertens

Megha Patwa

Adrián Villalobos

Simon Weiler

Maxim Wintergoller

Anna Dudek

Heike Faust

Sabrina Heide

Ansgar Jahn

Linh Pham


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Ricardo Castro

Hernandez


Last Name: Hernandez Position: Master/Bachelor Student
First Name: Ricardo Castro Location:
Academic Title: Tel.:

Publications

Curriculum Vitae


Publications

2019

RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.
Xie Y*, Hernandez RC*, Sokpor G, Pham L, Narayanan R, Rosenbusch J, Staiger JF, Tuoc T.
Molecular Neurobiology, https://doi.org/10.1007/s12035-019-1595-1, 2019.
abstract link

Chromatin remodeling factor BAF155 is an important regulator of many biological processes. As a core and scaffold subunit of the BAF (SWI/SNF-like) complex, BAF155 is capable of regulating the stability and function of the BAF complex. The spatiotemporal expression of BAF155 during embryogenesis is essential for various aspects of organogenesis, particularly in the brain development. However, our understanding of the mechanisms that regulate the expression and function of BAF155 is limited. Here, we report that RBM15, a subunit of the m6A methyltransferase complex, interacts with BAF155 mRNA and mediates BAF155 mRNA degradation through the mRNA methylation machinery. Ablation of endogenous RBM15 expression in cultured neuronal cells and in the developing cortex augmented the expression of BAF155. Conversely, RBM15 overexpression decreased BAF155 mRNA and protein levels, and perturbed BAF155 functions in vivo, including repression of BAF155-dependent transcriptional activity and delamination of apical radial glial progenitors as a hallmark of basal radial glial progenitor genesis. Furthermore, we demonstrated that the regulation of BAF155 by RBM15 depends on the activity of the mRNA methylation complex core catalytic subunit METTL3. Altogether, our findings reveal a new regulatory avenue that elucidates how BAF complex subunit stoichiometry and functional modulation are achieved in mammalian cells.

2018

Chromatin remodeling BAF155 subunit regulates the genesis of basal progenitors in developing cortex .
Narayanan R, Pham L, Kerimoglu C, Watanabe T, Hernandez RC, Sokpor G, Ulmke PA, Kiszka KA, Tonchev AB, Rosenbusch J, Seong RH, Teichmann U, Frahm J, Fischer F, Bonn S, Stoykova A, Staiger JF, Tuoc T.
iScience (Cell Press), DOI: https://doi.org/10.1016/j.isci.2018.05.014, 2018.
abstract link

The abundance of basal progenitors (BPs) - basal radial glia progenitors (bRGs) and basal intermediate progenitors (bIPs), in primate brain has been correlated to the high degree of cortical folding. Here we examined the role of BAF155, a subunit of the chromatin remodeling BAF complex, in generation of cortical progenitor heterogeneity. The conditional deletion of BAF155 led to diminished bIP pool and increased number of bRGs, due to delamination of apical RGs. We found that BAF155 is required for normal activity of neurogenic transcription factor PAX6, thus controlling expression of genes that are involved in bIP specification, cell-cell interaction and establishment of adherens junction. In PAX6-dependent manner, BAF155 regulates the expression of the CDC42 effector protein CEP4, thereby controlling progenitor delamination. Furthermore, BAF155-dependent chromatin remodeling seems to exert a specific role in the genesis of BPs through regulation of human RG-specific genes (such as Foxn4) that possibly acquired evolutionary significance.

ATP-dependent chromatin remodeling during cortical neurogenesis.
Sokpor G*, Hernandez RC*, Rosenbusch J, Staiger JF, Tuoc T.
Front. Neurosci. | doi: 10.3389/fnins.2018.00226 , 2018.
abstract link

The generation of individual neurons (neurogenesis) during cortical development occurs in discrete steps that are subtly regulated and orchestrated to ensure normal histogenesis and function of the cortex. Notably, various gene expression programs are known to critically drive many facets of neurogenesis with a high level of specificity during brain development. Typically, precise regulation of gene expression patterns ensures that key events like proliferation and differentiation of neural progenitors, specification of neuronal subtypes, as well as migration and maturation of neurons in the developing cortex occur properly. ATP-dependent chromatin remodeling complexes regulate gene expression through utilization of energy from ATP hydrolysis to reorganize chromatin structure. These chromatin remodeling complexes are characteristically multimeric, with some capable of adopting functionally distinct conformations via subunit reconstitution to perform specific roles in major aspects of cortical neurogenesis. In this review, we highlight the functions of such chromatin remodelers during cortical development. We also bring together various proposed mechanisms by which ATP-dependent chromatin remodelers function individually or in concert, to specifically modulate vital steps in cortical neurogenesis.



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