Dr. Mirko Witte
Researcher
![[Translate to Englisch:] Dr. Mirko Witte](/fileadmin/_processed_/d/8/csm_Witte_543e639551.jpg)
Dr. Mirko Witte
University Medical Center Göttingen
Center of Anatomy
Department of Neuroanatomy
Kreuzbergring 40, EG 0.122
D-37075 Göttingen
Phone: +49 551 3969876
E-mail: mirko.witte(at)med.uni-goettingen.de
Curriculum vitae
| Year | |
|---|---|
| Since August 2010 | Postdoc at the University Medical Center Göttingen of the Georg-August-University, Center of Anatomy, Department of Neuroanatomy under the direction of Prof. Dr. J. Staiger |
| 2010 | PhD in the Neurobiology Group at the University of Leipzig under the supervision of Prof. Dr. R. Rübsamen, topic: "Differentiation and maturation of GABAergic and glycinergic neurotransmission in the anteroventral cochlear nucleus of the gerbil (Meriones unguiculatus)" |
| 2008 - 2009 | research associate at the University of Leipzig in the neurobiology group of Prof. Dr. R. Rübsamen |
| April 2005 - April 2010 | Member of the International PhD Program (IPP) "From Signal Processing to Behavior" |
| April 2005 - April 2010 | Member of the Research Training Group GRK 1097/1 "Interneuro" |
| 2005 | Diploma thesis in the Neurobiology group at the University of Leipzig under the supervision of Prof. Dr. R. Rübsamen, topic: "Development of inhibitory influences in the cochlear nucleus: a patch-clamp study" |
| 2003 | Project work in the Behavioral Biology Group at the University of Leipzig under the direction of PD Dr. P. A. Stevenson and Prof. Dr. K. Schildberger, topic: "The aminergic modulation of the mandibular sphincter muscle in the cricket (Gryllus bimaculatus)" |
| 2001 - 2005 | Major studies in biology at the University of Leipzig with the main subjects: Neurobiology, Genetics, Behavioral Physiology, Special Zoology and Biochemistry |
| 1999 - 2001 | Basic studies in biology at the University of Leipzig |
Research
Functional analysis of networks of GABAergic interneurons in primary somatosensory (barrel) cortex
GABA-releasing interneurons are substantially involved in information processing in the neocortex and are directly involved in the somatosensory circuitry. Martinotti cells, a readily identifiable type of GABAergic interneuron, are thought to play a central role in modulating dendritic excitation of pyramidal cells because of their morphological structure. Thus, they possess a direct influence on the neocortical output structures of the barrel cortex. In addition to excitatory inputs from surrounding pyramidal cells, Martinotti cells are the target of marked behavioral inhibition, the neocortical origin of which is unclear. In this study, the cells of origin of inhibitory projections to Martinotti cells of neocortical layers 2/3 and 5 in barrel cortex will be investigated using combined whole-cell patch-clamp derivations and local photolysis of caged-glutamate in acute brain slices. Subsequently, "paired" patch-clamp derivations from Martinotti cells and their directly associated GABAergic interneurons will be used to uniquely identify and characterize these interneurons. The knowledge gained will contribute to the model of sensory information processing in the barrel cortex and improve the understanding of the networks of GABAergic interneurons.
Research Objectives:
- Patch-clamp recordings from Martinotti cells and identify the neocortical layers from which neurons inhibitively project to Martinotti cells using "caged-glutamate" photolysis.
- Establishment of the method to identify connected GABAergic interneurons in the selected (I) neocortical layers in acute brain slice.
- Simultaneous "paired" patch-clamp recordings from Martinotti cells and the upstream inhibitory interneurons, and their characterization according to the Petilla terminology.
Publications
2022
Repetitively burst-spiking neurons in reeler mice show conserved but also highly variable morphological features of layer Vb-fated “thick-tufted” pyramidal cells.
Jochen F. Staiger*, Alexandra Sachkova, Martin Möck, Julien Guy and Mirko Witte.
Front. Neuroanat. 16:1000107. doi: 10.3389/fnana.2022.1000107, 2022.
Abstract
2020
Increased Callosal Connectivity in Reeler Mice Revealed by Brain-Wide Input Mapping of VIP Neurons in Barrel Cortex.
Georg Hafner, Julien Guy, Mirko Witte, Pavel Truschow, Alina Rüppel, Nikoloz Sirmpilatze, Rakshit Dadarwal, Susann Boretius, Jochen F Staiger.
Cerebral Cortex, bhaa280, doi.org/10.1093/cercor/bhaa280, 2020.
Abstract
2019
Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs.
Hafner G, Witte M, Guy J, Subhashini N, Fenno LE, Ramakrishna C, Kim YS, Deisseroth K, Callaway EC, Oberhuber M, Conzelmann KK, Staiger JF.
Cell Reports 28 (13) P3450-3461.E8, 2019.
Abstract
Characterizing the morphology of somatostatin‐expressing interneurons and their synaptic innervation pattern in the barrel cortex of the GFP‐expressing inhibitory neurons mouse.
Zhou X., Mansori I., Fischer T., Witte M., Staiger JF..
J Comp Neurol. 2019;1–17, 2019.
Abstract
Neuromodulation Leads to a Burst-Tonic Switch in a Subset of VIP Neurons in Mouse Primary Somatosensory (Barrel) Cortex.
Prönneke A, Witte M, Möck M, Staiger JF.
Cerebral Cortex doi: 10.1093/cercor/bhz102, 2019.
Abstract
Distribution Patterns of Three Molecularly Defined Classes of GABAergic Neurons Across Columnar Compartments in Mouse Barrel Cortex.
Almási Z, Dávid C, Witte M, Staiger JF.
Frontiers in Neuroanatomy 13:45 doi: 10.3389/fnana.2019.00045, 2019.
Abstract
2017
Nicotine reverses hypofrontality in animal models of addiction and schizophrenia.
Koukouli,F.; Rooy,M.; Tziotis,D.; Sailor,K.A.; O'Neill,H.C.; Levenga,J.; Witte,M.; Nilges,M.; Changeux,J.P.; Hoeffer,C.A.; Stitzel,J.A.; Gutkin,B.S.; DiGregorio,D.A.; Maskos,U..
Nature Medicine, 2017.
Abstract
2016
Intracortical Network Effects Preserve Thalamocortical Input Efficacy in a Cortex Without Layers.
Guy,J.; Sachkova,A.; Möck,M.; Witte,M.; Wagener,R.J.; Staiger,J.F.
Cerebral Cortex DOI 10.1093/cercor/bhw281, 2016.
Abstract
Parvalbumin- and vasoactive intestinal polypeptide-expressing neocortical interneurons impose differential inhibition on Martinotti cells.
Walker F, Möck M, Feyerabend M, Guy J, Wagener RJ, Schubert D, Staiger JF, Witte M.
Nature Comunications 7:13664 (DOI: 10.1038/ncomms13664, 2016.
Abstract
2015
Thalamocortical Connections Drive Intracortical Activation of Functional Columns in the Mislaminated Reeler Somatosensory Cortex.
Robin J. Wagener, Mirko Witte, Julien Guy, Nieves Mingo-Moreno, Sebastian Kügler, Jochen F. Staiger.
Cerebral Cortex, 2015.
Abstract
Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences.
Prönneke A, Scheuer B, Wagener RJ, Möck M, Witte M, and Staiger JF.
Cereb. Cortex (2015) 25 (12): 4854-4868. doi: 10.1093/cercor/bhv202 , 2015.
Abstract
What types of neocortical GABAergic neurons do really exist?.
Jochen F. Staiger, Martin Möck, Alvar Prönneke, Mirko Witte .
e-Neuroforum (Springer), 2015.
Abstract
2014
Depolarizing chloride gradient in developing cochlear nucleus neurons: Underlying mechanism and implication for calcium signaling.
Witte M, Reinert T., Dietz B., Nerlich J., Rübsamen R., Milenkovic I..
Neuroscience 261: 207-222 , 2014.
Abstract
2010
Presynaptic and postsynaptic origin of multicomponent extracellular spike waveforms at the endbulb of held/spherical bushy cell synapse.
Typlt M., Haustein M., Dietz B., Steinert J., Witte M., Englitz B., Milenkovic I., Kopp-Scheinpflug C., Forsythe I., Rübsamen R..
Eur J Neuroscience 31(9):1574-81 , 2010.
Abstract
2009
P2 receptor-mediated signaling in spherical bushy cells of the mammalian cochlear nucleus.
Milenkovic, I., Rinke I, Witte M, Dietz, B., Rübsamen R..
J Neurophysiol. 102(3):1821-1833, 2009.
Abstract
2007
Development of chloride-mediated inhibition in neurons of the anteroventral cochlear nucleus of gerbil (Meriones unguiculatus).
Milenkovic I., Witte M., Turecek R., Heinrich M., Reinert T., Rübsamen R..
J Neurophysiol 98: 1634-1644, 2007.
Abstract
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